Abstract
In periodontitis, gingival fibroblasts undergo cytopathic degenerative changes. A variety of architectural components of Capnocytophaga strain S3 and fractions included lipids of Actinomyces naeslundii ATCC 12104 were analyzed for their ability to inhibit proliferation of human fibroblasts obtained from highly inflamed gingival margins.
A fraction solubilized with 1% sodium dodecyl sulfate from cell envelope of Capnocytophaga and a lipid fraction extracted solubly from A. naeslundii with chloroform-methanol (2:1, by volume) gave the greatest inhibitory activity against Fibroblast. Lipopolysaccharide fractions of Capnocytophaga and amphipathic substance of A. naeslundii as well as lipopolysaccharide of E. coli revealed less activities in growth inhibition. Peptidoglycan of Capnocytophage, however, exhibited little inhibitory activitiy. The results suggest that oral microorganisms possess architectural substance with no contamination of lipopolysaccharide and peptidoglycan that interfere with gingival fibroblast proliferation.
