Abstract
The investigations relating to humoral and cell-mediated immunity were carried out with 6 participants in a 3 week experimental gingivitis program. At the beginning of the experiment immediately, 1 and 3 weeks after oral hygiene withdrawal and 1 week after oral hygiene reinstitution, the bacterial plaque accumulation and the gingival inflammation were assessed prior to the gingival fluid measurement and blood taking. The effect of plaque accumulation upon the antibody forming cells (PFC) was examined using modified haemolytic plaque assay. The cytotoxicity of PBL to chicken erythrocytes were assessed using a modification of the standard 51Cr release assay. Serum 1gG and IgM concentrations by single radial immunodiffusion, and serum antibody titers to plaque antigen by haemagglutination test were evaluated, respectively.
In conclusion, the number of PFC and the cytotoxic activity in the plaque accumulation subjects were significantly increased compared with those in the subject with healthy gingiva. However, no significant differences in the concentrations of serum IgG and IgM and haemagglutinating titers to plaque antigen between two groups were observed.
