Abstract
The purpose of this study is to investigate the relation between herpes simplex virus and periodontal disease.
We collected 180 samples from non-operated human saliva, and 32 samples from saliva with periodontal disease at pre-operation, one and 7 days after operation, and furthermore collected gingiva during operation.
And then, from these samples we isolated herpes simplex virus by inoculation on tissue cultures of GMK cells (African green monkey kidney cells).
The results obtained are as follows:
1. Only one strain of herpes simplex virus was isolated from 180 samples of human saliva collected at Hospital of Kanagawa Dental College.
2. Herpes simplex virus were isolated with high rate from 32 samples of saliva in operated group as compared with 180 samples of saliva in nonoperated group.
3. 4 strains of herpes simplex virus were isolated from 32 samples of human gingiva with periodontal disease.
4. Herpes simplex virus isolated were determined as type 1 by plaque forming ability on CE (chick embryo) cells.
5. Consequently, it is suggested that herpes simplex virus is involved in periodontal disease.
