Abstract
The objective of this study was to search for evidence of antibody dependent cell-mediated cytotoxity (ADCC) that might relate to periodontal tissue destruction.
We investigate ADCC in vitro against fibroblastoid cells derived from human normal gingival tissue and the presence of K cells in infiltrated cells from human gingival tissues with periodontitis. Fibroblastoid cells binding Eikenella corrodens were lysed by normal human peripheral blood lymphocytes (PBL) after presensitization with rabbit antiserum to the bacteria. In infilterated cells obtained from the human inflamed gigival tissues by enzymatic treatment, Fc receptor (FCR) bearing cells were detected by IgG-EA rosettes formation. These cells, however, exhibited no ADCC activities against sheep erythrocytes as targets. Human PBL in positive control group and rat infiltrated cells from experimental inflamed lesion showed significant ADCC activities. But these cells did not mediate ADCC after enzymatic treatment, even though similar proportion of the cells bearing FcR before and after the treatment in human PBL.
