Abstract
The effect of in vivo administration of disodium ethan-l-hydroxy-1.1-diphosphonate (EHDP) on the mitogenic response of thymocytes or spleen cells were studied in young rats. When rats (2-3 weeks of age) were injected with EHDP (10mg/kg/day) subcutaneously for more than 6 days or injected once with EHDP (100mg/kg) subcutaneously or intraperitoneally, lipopolysaccharide-induced proliferative response of spleen cells were diminished. The in vivo administration of EHDP did not affected the proliferative response of thymocytes or spleen cells to concanavalin A. These results indicated that the in vivo administration of EHDP did induce the dysfunction of splenic B lymphocytes but not that of T lymphocytes.
