Abstract
This study was undertaken to determine the ability of some root filling materials to permeate the dentin of extracted teeth, and the effects of these root filling materials on healing after mucoperiosteal flap surgery.
In the first experiment, fifty-four freshly extracted dogs' teeth which were enlarged with hand reamers and files up to No. 70 were prepared as test pieces. The test pieces were filled with gutta-percha (Group 1) and Neotriozinc paste (Group 2) and the ends of the test pieces were sealed with wax. In the control group, the teeth were left empty. After the test pieces were tested by the agar diffusion method by placing them on a culture plate containing L-929 cells, and incubated for 24 hours. The dimensions of the clear colorless zones of dead cells around the test pieces were measured to determine their permeability.
In the second experiment, sixty mandibular premolars in mature dogs were examined. After pulp extirpation, the root canals were enlarged with hand reamers and files up to No. 70 in groups 1 and 2 (experimental group). The root canals were filled with warm gutta-percha in group 1, and with Neotriozinc paste in group 2. In the control group, the pulp of the teeth were not disturbed. Then mucoperiosteal flap surgery was carried out in all three groups. The animals were sacrificed at periods of 1, 2, 4, 8 and 12 weeks after the surgery, and examined histopathologically by light and electron microscopes.
The results were as follows:
1. In the first experiment, permeability of the extracted teeth to the materials was observed in group 2.
2. In the second experiment, the histologic findings showed that healing of the periodontium were delayed for one or two weeks after the surgery in the experimental group, and that healing was similar in the experimental groups and the control group 12 weeks after the surgery.
