Abstract
The purpose of this study was to develop an ELISA assay to chondroitin sulfate isomers (C4S, C6S and DS) proteoglycan in GCF associated with experimentally-induced periodontitis in dogs. Combination of these antibodies (9-A-2 and 3-B-3) and specific enzymatic digestion have made possible the identification of C4S, C6S and DS. Experimental periodontitis was induced by silk ligature placement below the gingival margin of the canine mandibular molars. GCF was collected in microcapillary tubes at 0, 3, 7, 21 and 60 days after ligature in three mongrel dogs. Samples were diluted 5. 10 in 0.15M PBS pH 7. 2. Indirect ELISA was performed. Standard curve and detection level of GAG-PG had been examined in a preliminary study using bovine nasal cartilage proteoglycan monomer. The quantity of C4S, C6S and DS, though fluctuating, increased in proportion with the severity of inflammation. In particular, C4S was increased in the acute phase and then decreased gradually. The present study has confirmed that ELISA assay enables the detection of GAGs in GCF associated with experimentally-induced periodontitis in the dog.
