Abstract
This study was performed to know the best way of some periodontal treatments on the diseased root cementum by attachment and growth of cultured human gingival fibroblasts.
Four modalities of root treatments were performed as experimental groups: 1) ultrasonic scaling, Sc. 2) polishing by rubber cup and paste followed by scaling, Sc + Po. 3) shaved 20μm surface layer of cementum by curette scaler followed by scaling, Sc + 20μRp. 4) removed all cementum was by curette type scaler followed by scaling (throughly root planing), Sc + Rp. Three groups were made as controls: 1) no treatment of diseased cementum 2) throughly root planing of healthy teeth 3) no treartment of healthy teeth.
Root fragments were prepared from surgically extracted human healthy and periodontally involved teeth and each tublet had 3 mm diameter and 1 mm thickness. Each group had 10 tublets. The treated root fragments were placed in petridishes and seeded 1 x 105/m/l fibroblasts in E-MEM containing 200 units/ml penicillin and 200 μm/m/l streptomycin followed by incubation for 72 h. After the tublets were fixed and stained, the number of attachments and growth cellls were counted by light microscopy and examined the morphologies by S. E. M.
The resutls were as follows:
1) the number of fibroblasts on the Sc-tublets was a few and their shapes were not enough to spread. 2) the number of cells attached on the Sc + Po-tublets was a few, but more than Sc group. 3) the number of cells attached on Sc + 20μRp and Sc + Rp groups was increased to the control level (C and C-Rp groups). Their cells were shaped flat and spreading, and they had many projections connecting to other cells.
These observations suggested that the treatments of shaving 20μm on the root surface and removing all cementum (throughly root planing) gave a good condition for attachment and growth of fibroblasts.
