Abstract
Actinobacillus actinomycetemcomitans is thought to play an important role in the pathogenesis of localized juvenile periodontitis (LJP), rapidly progressive periodontitis and recurrent periodontitis. A total of 38 clinical strains of Actinobacillus actinomycetemcomitans, freshly isolated from an adult periodontitis patient with diabetes mellitus, were examined in this study.
This study evaluated serological and biochemical characteritics of those clinical strains and compared them with those of ATCC 29523, Y 4 and NCTC 9710. These clinical strains of Actinobacillus actinomycetemcomitans had distinct colonial characteristics and positive catalase reactions on TSBV medium. All strains had sticky and cohesive colonial characteristics.
They were serologically characterized with the rabbit antiserum against to the NCTC 9710 type strain; ATCC 29523 and Y 4 reference strains Cell surface polysaccharide antigen was extracted from whole cells of Actinobacillus actinomycetemcomitans by autoclaving. Using serotype - specific antiserum, we identified three dintinct serotypes, a, b and c by the Ouchterlony agar immuno diffusion method.
Biotypes were characterized on the basis of the variable fermentation of 8 sugars on PY medium and identified by the Rap ID NH system (AMUCO, Tokyo). All strains were able to ferment D-Mannose, Levurose (D- (-) Fructose), Galactose, D (+) -Xylose and identified serotype a . They have been identified as Code number “2102” with the Rap ID NH system.
This patient harbored only one biotype and serotype of Actinobacillus actinomycetemcomitans.
