Abstract
In this study, we immunohistologically detected plasma cells bearing immunoglobulins reactive to human γ A-globulin (IgG) in gingivae from patients with various types of periodontitis. In order to detect these cells, we developed an immunohistological method using human IgG conjugated with horseradish peroxidase (HRP). 41 subjects were grouped into 3 different types of periodontal disease; adult periodontitis (AP), early onset periodontitis (EOP) and hyperplastic gingivitis (HG), according to their histories and clinical findings. On analysis of all 63 specimens from 41 subjects, plasma cells bearing immunoglobulins reactive to IgG were detected in 14 specimens from 9 subjects. These cells were observed in all groups and their existence ratios increased in the order of AP, HG and EOP. In almost all specimens in which plasma cells bearing immunoglobulins reactive to IgG were detected, these cells existed in the subepithelial connective tissues where plasma cells and lymphocytes tended to form clusters. In this study, it was unclear whether the existence of these cells related to clinical parameters. But the presense of many plasma cells bearing immunoglobulins reactive to IgG was confirmed in some phases of early onset periodontitis, suggesting that specific antibodies to human IgG are produced in gingiva affected by human periodontitis.
