Abstract
We have investigated the effects of periodontal ligament cells on osteocalstic bone resporption activity in cases of tooth replantation.
Human periodontal ligament Cells (Pla) were isolated from impacted wisdom teeth and proliferated in culture dishes. Pla was seeded on bovine bone slices for 24 h in a-MEM supplemented with 10% FCS. Isolated rabbit osteoclasts were added to Pla coated bone slices and cultured another 48 h under the same conditions. Bone slices were swiped, to remove cells, and stained immunochemically using anti-collagen type I antibody to visualize the resorption lacunae. The number and area of lacunae were quantitated microscopically with a digital image analyzer.
An other experiment was designed to study the effects of components of Pla on osteoclastic bone resorption activity. Osteoclasts attached to bone slices were placed at the bottom of a chamber which had been Pla cultured for 24 h above a partition membrane, and co-cultured for another 48 h.
In pre-seeded with 10' cells group, the number and area of resorption lacunae was significantly decreased as compared to the control. In the co -culture using a partition chamber, no significant differences were found among the groups.
These findings suggest that periodontal ligament cells can inhibit osteoclastic bone resorption activity under contact culture conditions.
