Abstract
The objective of this study, was quantitatively examine the effects of Ultrasonic extracts of Prevotella intermedia (P. intermedia). On the phagocytosis by polymorphonuclear leukocytes (PMN) which are major components of the host defense, and the extent of expression of Fcγ and C 3 bi receptors on the cell membrane surface was also studied with a Flow cytometer using PMNs from healthy subjects.
We obtained the follwing results.
1. The ultrasonic extracts of P. intermedia was decreased the phagocytic activity of PMNs in a dose dependent manner.
2. The extent of expression of Fcγ receptors on the cell membrane surface was reduced by stimula-tion of the ultrasonic extract, showing a significant correlation with the phagocytic activity of the cells.
3. The extent of expression of C3 bi receptors on the cell membrane surface was raised by stimula-tion of the ultrasonic extract, indicating a signifi-cant correlation with the phagocytic activity of the cells.
4. Heat treatment at 100°C for 30min or treat-ment with Proteinase K caused the extract to lose its effect on the phagocytic activity and the expres-sion of the receptors.
In conclusion, the ultrasonic extract of P. inter-media was suggested to change the functions of PMN and affect the receptor expression on the cell membrane surface, there by having a close affinity to periodontal disease.
