Abstract
The association of heparan sulfate proteoglycan (HSPG) with osteoclasts has not been clearly established. We have studied the immunohistochemical localization of HSPG using a specific monoclonal antibody against HSPG in osteoclast-rich tissues. Periodontal tissues from beagle dogs in which periodontitis had been induced with silk ligature and radii from newborn rabbits were used. Tissue samples were embedded in paraffin and sectioned. Immunoperoxydase staining of sections was performed using an anti-HS monoclonal antibody (HepSS-1) and biotin streptavidin. These sections were also stained for tartrate-resistant acid phosphatase (TRAP) to localize osteoclasts. In periodontal tissues, bone resorbing TRAP-positive multinucleated cells as well as TRAP-positive mononuclear cells located close to alveolar bone showed HSPG-positive cytoplasmic staining. In rabbit radii, the cytoplasm of TRAP-positive mononuclear and multinucleated, as well as, TRAP-negative cells stained positive. Whereas, sections pretreated with heparitinase as a control did not stain. In addition, isolated rabbit osteoclasts were cultured in medium-199 for 48 hours at 37... Immunocytochemical studies using HepSS-1 was performed for cultured osteoclasts. Laser confocal microscopic observation showed HSPG-positive staining located in the cytoplasm of osteoclasts. These observations indicate that HSPG is associated with the osteoclastic cell lineage. J. Jpn. Soc. Periodontol., 41: 229-237, 1999.
