Abstract
We studied the effects of basic fibroblast growth factor (b-FGF) and carriers on heterogenetic bone formation in subcutaneous tissues of rats using hybrid materials compounded with osteogenetic cells, which were first subcultured cells of rat bone marrow, and 3 types of carrier. We evaluated the optimal concentration of b-FGF needed to differentiate rat bone marrow cells in to osteoblasts. We measured the number of cells, alkaline phosphatase (ALP), and calcium concentration in cultured cells after 0, 2, 4, 6, 8, and 14 days of culture using 2.5 ng/ml and 25 ng/ml b-FGF. In the presence of 2.5 ng/ml b-FGF, ALP activity and calcium concentration were stimulated more than with 25 ng/ml b-FGF.
Hybrid materials compounded with osteogenetic cells and hydroxyapatite (HAP), true bone ceramic (TBC), and BioOss® were cultured for 2 weeks with and/or without 2.5 ng/ml b-FGF. These were incubated in PBS for 3 hours before implantation, and implanted into the subcutaneous tissues of rats. At 5 and 8 weeks after implantation, these samples were retrieved and examined histologically. In the hybrid materials of HAP, we observed better bone formation and maturation in the test group than in controls. Hybrid TBC and BiuOss® materials, however, did not reveal good bone formation in either the test group or controls. Results indicate that hybrid material using of 2.5 ng/ml b-FGF and HAP increases bone forming activity. J. Jpn. Soc. Periodontol., 43: 52-64, 2001.
