Abstract
Our previous study showed that electro-acupuncture (EA) pretreatment protects the heart from injury of ischemia. The present study explored further whether adenylate cyclase (AC), protein kinase A (PKA) and L-type Ca2+ channel, the β1-AR signaling components modulating intracellular Ca2+ ([Ca2+]i), are involved in the mediation of the anti-arrhythmic effect of EA pretreatment in the rats from which the hearts were subsequently isolated and subjected to simulative global ischemia and reperfusion (SGIR). SGIR was performed by perfusing the isolated heart at a low flow followed by normal perfusion. Adult rats were randomized into four groups, namely, normal control (NC), SGIR, EA and NC plus EA (NCEA) groups. The rats in EA and NCEA groups were given EA pretreatment at bilateral Neiguan points (PC6) for 30 min once a day in 3 consecutive days before the hearts were isolated and perfused. The arrhythmia score and the response of [Ca2+]i to the activators of AC, PKA and L-Ca2+ channel in single ventricular myocyte isolated from the hearts subjected to SGIR were compared among the groups. The results showed that the arrhythmia score was significantly higher in SGIR group as compared with NC and NCEA groups. The SGIR-enhanced arrhythmia score was significantly attenuated in EA group. More interestingly, EA pretreatment also attenuated SGIR-enhanced response of [Ca2+]i to the activators of AC, PKA and L-type Ca2+ channel in the myocytes isolated from the hearts subjected to SGIR. In conclusion, EA pretreatment can produce anti-arrhythmic effect in the rat of SGIR and AC, PKA and L-type Ca2+ channel are involved in the mediation of the anti-arrhythmic effect of EA pretreatment.
