Abstract
Tissue homogenates prepared from the adult stickleback, Pungitius pungitius, were electrophoresed on cellulose acetate sheets at pH 8.6 under a constant current of 3.0mA for 45 minutes at room temperature. After electrophoresis, LDH appears as a single, identical band in skeletal muscle, heart, and in a majority of tissues; two additional isozymes are found in both eye and brain homogenates. Moreover, the eye is unique in possessing three negatively charged isozymes in addition to the three cathodal varieties. These findings suggest that the stickleback should be classified as a three-isozyme fish with additional three minor isozymes appearing only in the eye.
