Reproduction of Sedum drymarioides, an Endangered Rare Species, by Micropropagation

Abstract

Sedum drymarioides, an endangered plant, was micro-propagated, and the regenerated plants showed the CO₂ exchange pattern of a C₃-plant. Stem and leaf explants were cultured on solid MS medium containing various concentrations of 1-naphtaleneacetic acid (NAA) and 6-benzylaminopurine (BAP), resulting in callus formation at 0.1-1mgl^-1 NAA. From the stem callus, the best differentiation of root and bud was observed at 1mgl^-1 of NAA plus 0.1mgl^-1 of BAP and 0.1mgl^-1 of NAA plus 1 mgl^-1 of BAP, respectively. From the leaf callus, the differentiation was most efficient with the combination of 1mgl^-1 of NAA plus 1mgl^-1 of BAP and 1mgl^-1 of NAA plus 10mgl^-1 of BAP, respectively. The buds further multiplied on medium containing 0.1mgl^-1 of NAA and 10mgl^-1 of BAP. They developed into rosette plantlets and grew to normal size on phytohormone-free MS medium in glass bottles, where they bolted and flowered. The regenerated plantlets were successfully transferred to pots where fertile seeds were obtained.