Plant Biotechnology
Online ISSN : 1347-6114
Print ISSN : 1342-4580
ISSN-L : 1342-4580
Gene technology
High-throughput functional screening of plant and pathogen genes in planta
Ryohei TerauchiKhairun Hisam Bin NasirAkiko ItoHiromasa SaitohThomas BerberichYoshihiro Takahashi
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2005 Volume 22 Issue 5 Pages 455-459

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Abstract

Understanding of molecular mechanisms of pathogenicity of Agrobacterium and plant viruses has allowed us to utilize these pathogens as efficient vectors for transgene expression in plants. These vectors are now employed for high-throughput overexpression screening of plant and pathogen genes in planta. Virus-induced gene silencing (VIGS) has become one of indispensable tools for functional analysis of genes. VIGS is also suitable for high-throughput screening of plant genes. In this review, we describe: (1) vector-mediated transient expression techniques; (2) high-throughput overexpression screening of pathogen genes, of which products elicit hypersensitive response (HR) on host plants; (3) high-throughput overexpression screening of plant genes that are related to HR-like cell death; and (4) high-throughput VIGS screening of plant genes necessary for HR.

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© 2005 by Japanese Society for Plant Cell and Molecular Biology
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