In vitro node culture of seedlings in bamboo plant, Phyllostachys meyeri McClure

Abstract

Phyllostachys meyeri cultivated in the Fuji Bamboo Garden, Japan showed monocarpic mass flowering from 2004 through 2005. We first examined in vitro germination of the caryopses and found that sterilized water supplemented with 0.1% Plant Preservative Mixture^TM was effective for their growth with an efficient germination rate (75%). Seedlings were transferred to a liquid modified half strength Murashige and Skoog (m1/2MS) medium for further growth and were acclimatized in soil mixture under ex vitro condition. Nodal segments which were collected from ca. 1.5 year-old seedlings and 1 year-old tissue cultured clone plants were re-sterilized and then cultured in a liquid m1/2MS medium. Shoot elongation with rooting could be seen within 8 weeks and they were successfully multiplied under ex vitro condition.