Plant Biotechnology
Online ISSN : 1347-6114
Print ISSN : 1342-4580
ISSN-L : 1342-4580
Direct introduction of neomycin phosphotransferase II protein into apple leaves to confer kanamycin resistance
Keiji NumataYoko HoriiYoko MotodaNarumi HiraiChikako NishitaniSatoru WatanabeTakanori KigawaYutaka Kodama
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2016 Volume 33 Issue 5 Pages 403-407


The recent developments of transcription activator-like effector nucleases (TALEN) and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 nuclease (Cas9) have expanded plant breeding technology. One technical issue related to the current genome editing process is residual transgenes for TALEN and CRISPR/Cas9 left in plant genomes after the editing process. Here, we aim to add transient kanamycin resistance into apple leaf cells by introducing neomycin phosphotransferase II (NPTII) into apple leaf cells using the fusion peptide system. At 75 mg/L of kanamycin for 2 days, apple JM1 leaf cells infiltrated with NPTII could be selected. Thus, we successfully demonstrated the first transient selection system of plant cells using a fusion peptide-mediated protein delivery system.

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© 2016 by Japanese Society for Plant Biotechnology
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