Abstract
The effect of sampling positions and plant growth regulators on shoot and bulblet formation in vitro was investigated, using bulbs or bulbils of garlic plant, Allium sativum L.
When shoot tips were explanted, the number of shoots formed increased in higher benzyladenine (BA) concentrations. Bulblet formation increased in either low BA or naphthalene-acetic acid (NAA) concentrations, and increased most in hormone free medium. When basal plates were explanted, the higher the concentrations of BA, the more shoot formation tended to be promoted. When the lower parts of the foliage leaves were explanted, the higher the concentrations of BA, the more shoot formation was promoted. The number of shoots formed increased more than for any other sampling position. Bulblet formation occurred in the plots that contained either BA or NAA.