Effects of Sampling Dates of Runners on Plantlet Growth in Shoot Apex Culture of Strawberry (Fragaria×ananassa Duchesne)

Abstract

The effects of sampling timing of runners on plantlet growth were examined in shoot apex culture of strawberry ‘Belle rouge’.
Those plantlets sampled from June 23 to Aug. 4 differed in development from those plantlets sampled from Aug. 18 to Sept. 21. The plantlets sampled at the late term had fewer developed leaves and greater plant heights than those sampled at the early term.
Auxin activities of apical meristems of stock plants were examined using Avena bioassay. The auxin activity was highest on the first date of sampling and then declined. These results suggested that shoot apex excised at the early term would be desirable for in vitro culture.