Molecular Identification of Two Genes of Germin-like Protein in Arabidopsis

Abstract

We identified two homolog genes of PnGLP (Pharbitis nil germin-like protein) and SaGLP (Sinapis alba GLP) in Arabidpsis thaliana, namely, AtGLP1 and AtGLP2. Results of Southern blotting showed that each gene was single per haploid genome. Northern blotting revealed the presence of mRNAs for both genes in all overground parts, but especially in the leaf and flower. RFLP mapping showed that AtGLP1 was on chromosome 1 (112.4±0.6cM) and AtGLP2 was on chromosome 5 (35.7±0.3cM).