In vivo live-cell imaging in plant tissues by two-photon excitation microscopy

Abstract

In vivo live-cell imaging is an essential for understanding biological phenomena. In biological science, recent advances in imaging techniques using various fluorescence probes revealed dynamic cellular/extracellular events as they occur in real time at subcellular level. Two-photon excitation microscopy provides non-invasive tool for deep imaging in living organisms. We have been trying to deep imaging in plant tissues, using two-photon excitation microscopy to study the biological phenomena in vivo. In this review article, we summarize the characters of two-photon microscopy and advantages for deep imaging of plant tissues in vivo.