Two-photon spinning disk confocal microscopy of living cells and tissues

Abstract

Analyses of cellular and subcellular dynamics in living cells and tissues with their three-dimensional (x, y, z) structural information are superior to those with single-plane information. Two-photon spinning disk confocal microscopy is a good method for taking spatially and temporally high-resolution images for reconstruction of three-dimensional view. By using this method, we expect elucidation of dynamics of intracellular structures in a tissue, such as microtubule dynamics in cells inside of a root apical meristem.