Online ISSN : 1884-4154
Print ISSN : 0918-9726
ISSN-L : 0918-9726
Invited Review (Special Feature I)
Quantitative evaluation of cytoskeletal organizations by microscopic image analysis
Takumi Higaki
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2017 Volume 29 Issue 1 Pages 15-21


Visualization of cytoskeletal organizations is a fundamental research method for understanding plant cell activities. Conventionally, immunostaining methods have been used to visualize cytoskeletons, but some technical difficulties make it hard to obtain a large number of reliable microscopic images. The introduction of fluorescent protein tagging technology and the development of high-throughput method for microscopic image acquisition have made it easier and quicker to obtain many reliable microscopic digital images of cytoskeletons. Based on these technical improvements, a method for quantitative evaluation of cytoskeletal organizations by image analysis has been developed. This method has become an indispensable research approach in state-of-the-art plant cell biology. In this minireview, I outline a practical method to measure image features to quantitatively evaluate the orientation, parallelness, bundling, and density of cytoskeletons using the ImageJ image analysis software.

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© 2017 The Japanese Society of Plant Morphology
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