2021 Volume 33 Issue 1 Pages 15-23
Morphology of plants is controlled by coordinated patterns of cell division and growth. Understanding the molecular mechanisms that regulate plant architecture requires careful observation and detailed analysis at the level of cells and tissues. In this review, we introduce recently developed microscopy protocols and computational tools to obtain and analyze cellular level data for research on plant morphology and development. We mainly focus on protocols for imaging plant structures by confocal laser scanning microscopes, followed by quantitative analysis based on 3D segmentation of cells layers/tissues, and computational modelling to explore fundamental questions in plant developmental biology.
