2025 Volume 37 Issue 1 Pages 27-32
Most land plants grow roots in the soil and interact with various soil microorganisms throughout their life. Legumes, such as soybeans, form symbiotic structures called root nodules through interaction with rhizobia. Microscopic observations have advanced our understanding of rhizobial infection processes. However, previous research has primarily relied on the destructive method of excavating roots from the soil. In this paper, we introduce the Rhizosphere Frame system, which we have developed for nondestructive observation of root nodule symbiosis in the soil. This system is expected to facilitate a deeper understanding of the dynamics of plant-microbe interactions in the soil environment.
