Behavior of mitochondria in synchronized cells of Chlamydomonas reinhardtii

Tomoko Ehara

doi:10.5685/plmorphol.10.50

Tomoko Ehara

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Keywords: Chlamydomonas reinhardtii, synchronized culture, giant mitochondrion, behavior of mitochondria

JOURNAL FREE ACCESS

1998 Volume 10 Issue 1 Pages 50-59

DOI https://doi.org/10.5685/plmorphol.10.50

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Published: 1998 Received: - Available on J-STAGE: June 28, 2010 Accepted: - Advance online publication: - Revised: -
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Date of correction: June 28, 2010 Reason for correction: - Correction: PDF FILE Details: -

Date of correction: June 28, 2010 Reason for correction: - Correction: KEYWORD Details: Wrong : Key words: Chlamydomonas reinhardtii, synchronized culture, giant mitochondrion , behavior of mitochondria
Right : Chlamydomonas reinhardtii, synchronized culture, giant mitochondrion, behavior of mitochondria

Date of correction: June 28, 2010 Reason for correction: - Correction: CITATION Details: Wrong : E
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Right : Arnold C. G., Schimmer O., Schö tz F.und Bathelt H. (1972)Variety of mitochondrial shapes, sizes and volumes in Chlamydomonas reinhadtii. Arch.Mikrobiol., 81: 50-67.
Bereiter-Hahn J. (1976)Dimethylaminostyrylmethylpyridiniumiodine(DASPMI)as a fluorescent probe for mitochondria in situ. Biochim. Biophy. Acta, 423: 1-14.
Blank R. and Arnold C. G. (1980b)Variety of mitochondrial shapes, sizes and volumes in Chlamydomonas reinhardtii. Protoplasma, 104: 197-191.
Brandt P. (1975)Zwei Maxima plastidarer DNA-Synthese mit unterschiedlicher Lichtabhangigkeit im Zellzyklus von Euglena gracilis. Planta, 124: 105-107.
Calvayrac R., Butow R. A. and Lefort-Tran M. (1972)Cyclic replication of DNA and changes in mitochondrial morphology during the cell cycle of Euglena gracilis(Z). Exptl. Cell Res., 71: 422-432.
Chiang K. S. and Sueoka N. (1967)Replication of chloroplast DNA in Chlamydomonas reinhardtii during vegetative cell cycle: its mode and regulation. Proc. Natl. Acad. Sci. USA, 57: 1505-1513.
Cook J. R. (1966a)The synthesis of cytoplasmic DNA in synchronized Euglena. J. Cell Biol., 29: 369-373.
Ehara T., Osafune T. and Hase E. (1985)Interactions between the nucleus and cytoplasmic organelles during the cell cycle of Euglena gracilis in synchronized cultures III. Associations between the nucleus and chloroplasts at an early stage in the cell cycle under photoorganotrophic conditions(PartII)Plant Cell Physiol., 26: 1155-1165.
Ehara T., Ogasawara Y., Osafune T. and Hase E.(1990a)Behavior of chloroplast nucleoids during the cell cycle of Chlamydomonas reinhardtii(chlorophyta)in synchronized culture. J. Phycol., 26: 317-323.
Ehara T., Osafune T. and Hase E. (1990b)Interactions between the nucleus and cytoplasmic organelles during the cell cycle of Euglena gracilis in synchronized cultures IV.An aggregate form of chloroplasts in association with the nucleus appearing prior to chlo roplast division. Exptl. Cell Res., 190: 104-112.
Ehara T., Osafune T. and Hase E. (1995)Behavior of mitochondria in synchronized cells of Chlamydomonas reinhardtii(Chlorophyta). J. Cell Sci. 108: 499-507.
Ehara, T., Osafune T. and Hase E. (1996)Association of nuclear and chloroplast DNA molecules in synchronized cells of Chlamydomonas reinhardtii. J. Electron Microsc., 45: 159-162
Kuroiwa, T. (1982)Mitochondrial nuclei, Int. Rev. Cytol., 75: 1-59.
Mihara S. and Hase E. (1971)Studies on the vegetative life cycle of Chlamydomonas reinhardtii Dangeard in synchronous culture I. Plant Cell Physiol., 12: 225-236.
Nass M. M. K. and Nass S. (1962)Fibrous structures within the matrix of developing chick embryo mitochondria. Expl, Cell Res., 26: 424-437.
Osafune T. (1973)Three-dimensional structures of giant mitochondria, dictyosomes and“ concentric lamellar bodies” formed during the cell cycle of Euglena gracilis(Z)in synchronized culture. J. Electron Microsc., 22: 51-61.
Osafune T., Mihara S., Hase E. and OhkuroI. (1975)Formation and division of giant mito chondria during the cell cycle of Euglena gracilis Z in synchronous culture I. Some characteristics of changes in the morphology of mitochondria and oxygen-uptake activity of cells. Plant Cell Physiol., 16: 313-326.
Osafune T., Ehara T., Hase E.and Schiff J. A. (1993)Stage-dependent association of nuclear and chloroplast DNA molecules through bridges transiently farmed between the two organelles in synchronized cells of Euglena gracilis. J. Electron Microsc., 42: 197-201.
Pellegrini M. (1980)Three-dimensional reconstruction of organelles in Euglena gracilis Z. I. Qualitative and quantitative changes of chloroplasts and mitochondrial reticulum in synchronous photoautotrophic culture. J. Cell Sci., 43: 137-166.
Ryan R. D., Grant K., Chiang S. and Swift H. (1978)Isolation and characterization of mito chondrial DNA from Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci. 75: 3268-3272
Tamiya H. (1966)Synchronous cultures of algae. Ann. Rev. Plant Physiol., 17: 1-26.
Tsukada S., Ehara T., Sumida S., Osafune T. and Hase E. (1986)Behaviour of proplastids and their nucleoids in dark-organotrophically grown cells of Euglena gracilis transferred to an inorganic medium. Br. Phycol. J., 21: 155-163.

Abstract

Cells of Chlamydomonas reinhardtii Dangeard were synchronized under a 12 hour light: 12hour dark regimen.Behavior of mitochondria in these synchronized cells was examined by fluorescence microscopy using a mitochondrial membrane-binding fluorescent dye, DASMPI, as a specific vital stain as well as by electron microscopy. Three-dimensional models of mitochondria in synchronized cells of C. reinhardtii were constructed from their electron microscopic profiles in serial cell sections using the computer graphics techniques. Based on these observations, the mitochondrial morphologies of C. reinhardtii in the cell cycle were classified into five types. These results were discussed in relation with those reported by previous workers.

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