Ultrastructure of Germinating Pea Leaves Prepared by High-Pressure Freezing

Yasuko Kaneko

doi:10.5685/plmorphol.12.10

Yasuko Kaneko

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Keywords: seed germination, Pisum sativum, ultrastructure, high-pressure freezing, lipid bodies

JOURNAL FREE ACCESS

2000 Volume 12 Issue 1 Pages 10-19

DOI https://doi.org/10.5685/plmorphol.12.10

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Published: 2000 Received: - Available on J-STAGE: June 28, 2010 Accepted: - Advance online publication: - Revised: -
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Date of correction: June 28, 2010 Reason for correction: - Correction: PDF FILE Details: -

Date of correction: June 28, 2010 Reason for correction: - Correction: KEYWORD Details: Wrong : Key Wbrds: seed germination; Pisum sativum; ultrastructure; high-pressure freezing; lipid bodies
Right : seed germination, Pisum sativum, ultrastructure, high-pressure freezing, lipid bodies

Date of correction: June 28, 2010 Reason for correction: - Correction: CITATION Details: Wrong : E
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Abstract

Summay. High-pressure freezing-freeze substitution methods(HPF-FS)were applied to germinating pea leaves. Good ultrastructural preservation without visible freezing damage was obtained up to 200μm in thickness. Compared to conventional chemical fixation(CF), cellular membranes were smoother without undulation, and organelles appeared more turgid. The matrices of cytoplasm and organelles were denser and more homogeneous. These features imply that HPF-FS samples retain more substances and ultrastructure closer to the living state. There were differences in membrane stainability among organelles in HPF-FS specimens, which were not seen after CF. Bundles of microfilaments were observed frequently after HPF-FS. Dynamic ultrastructural change during the first 24h of imbibition of dry seeds was revealed by this method.

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