Chromosome analysis by fluorescence banding and in situ hybridization in gymnosperms

Masahiro Hizume

doi:10.5685/plmorphol.12.52

Masahiro Hizume

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Keywords: chromosome, , ,, fluorescent banding, in situ hybridization, gymnosperm

JOURNAL FREE ACCESS

2000 Volume 12 Issue 1 Pages 52-59

DOI https://doi.org/10.5685/plmorphol.12.52

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Published: 2000 Received: - Available on J-STAGE: June 28, 2010 Accepted: - Advance online publication: - Revised: -
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Date of correction: June 28, 2010 Reason for correction: - Correction: PDF FILE Details: -

Date of correction: June 28, 2010 Reason for correction: - Correction: KEYWORD Details: Wrong : Key words: chromosome, fluorescent banding, in situ hybridization, gymnosperm
Right : chromosome, , ,, fluorescent banding, in situ hybridization, gymnosperm

Date of correction: June 28, 2010 Reason for correction: - Correction: CITATION Details: Wrong : E
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Right : Brown, G. R., Newton, C.H. and Carlson, J.E.(1998)Organization and distribution of a Sau3A tandem repeated DNA sequence in Picea (Pinaceae) species. Genome 41: 560-565.
Hizume, M.(1988)Karyomorphological studies in the family Pinaceae. Mem. Fac. Educ. Ehime Univ., Ser. III Nat. Sci. 8: 1-108.
Hizume, M., Abe, K. K. and Tanaka, A.(1988)Fluorescent chromosome bandings in Taxodiaceae. La Kromosomo II-50: 1609-1627.
Hizume, M., Arai, M. and Tanaka, A.(1990)Chromosome banding in the genus Pinus. III. Fluorescent banding pattern of P. luchuensis and its relationships among the Japanese diploxylon pines. Bot. Mag. Tokyo 103: 103-111.
Hizume, M., Fujii, S., Kondo, K., Gu, Z. and Yue, Z.(1993a)Fluorescent bandings in Ketelerria evelyniana and K. davidiana var. formosana, Pinaceae. La Kromosomo II-71-72: 2443-2450.
Hizume, M., Gu, Z., Yue, Z. and Kondo, K.(1994a)Fluorescent chromosome banding in Larix potaninii var. macrocarpa collected in Yunnan, China. In Proceedings of 2nd Sino-Japanese Sympodium on Plant Chromosomes(Plant chromosome Research 1992). edited by R. Tanaka. International Academic Publishers, Beijing. pp 45-50.
Hizume, M., Ishida, F. and Murata, M.(1992)Multiple locations of ribosomal RNA genes in chromosomes of pines, Pinus densillora and P. thunbergii. Jpn. J. Genet. 67: 389-396.
Hizume, M., Kitazawa, N., Gu, Z. and Kondo, K.(1991)Variation of fluorescent chromosome band in Picea brachytyla var. complanata collected in Yunnan, China. La Kromosomo II-63-64: 2149-2158.
Hizume, M. and Kondo, K.(1992)Fluorescent chromosome banding in five taxa of Pseudotsuga, Pinaceae. La Kromosomo II-66: 2257-2268.
Hizume, M., Kondo, K., Zhang, S. and Hong, D.(1998a)Fluorescence chromosome banding in a Chinese larch, Larix chinensis Beissn. Chromosome Science 2: 95-98.
Hizume, M. and Kondo, T.(2000)Relatihonships between fluorescent chromosome bands and DNA sequences in conifers. In Cytogentics studies of Forest Trees and Schrubs. Ed. Guttenberger H. et al., Graz, pp. 71-79.
Hizume, M., Kurose, N., Shibata, F. and Kondo, K.(1998b)Molecular cytogenetic studies on sex chromosomes and proximal heterochromatin containing telomere-like sequence in Cycas revoluta. Chromosome Science 2: 63-72.
Hizume, M., Kuzukawa, Y., Kondo, K., Yang, Q., Hong, D.-Y. and Tanaka, R.(1995)Localization of rDNAs and fluorescent bandings in chromosomes of Larix potaninii var. macrocarpa collected in Sichuan, China. La Kromosomo II-78: 2689-2694.
Hizume, M., Ohgiku, A. and Tanaka, A.(1983)Chromosome banding in the genus Pinus. I. Identification of chromosomes in P. nigra by fluorescent banding method. Bot. Mag. Tokyo 96: 273-276.
Hizume, M., Ohgiku, A. and Tanaka, A.(1989)Chromosome banding in the genus Pinus. II. Interspecific variation of fluorescent banding patterns in P. densiflora and P. thunbergii. Bot. Mag. Tokyo 102: 25-36.
Hizume, M, Shibata, F., Kondo, K, Hoshi, Y., Kondo, T., Ge, S., Yang Q. and Hong D.(1999)Identification of chromosomes in two Chinese spruce species by multicolor fluorescence in situ hybridization. Chromosome Science 3: 37-41.
Hizume, M., Shiraishi, H. and Tanaka, A.(1988)A cytological study of Podocarpus macrophyllus with special reference to sex chromosomes. Jpn. J. Genet. 63: 413-423.
Hizume, M. and Tanaka, A.(1990)Fluorescent chromosome bandings in two American larches, Larix occidentalis and L. laricina. La Kromosomo II-58: 1979-1987.
Hizume M., Tominaga K. and Tanaka A. 1988. Fluorescent chromosome banding in Larix leptolepis(Pinaceae). Bot. Mag. Tokyo 101: 333-336.
Hizume, M., Tominaga, H. H., Kondo, K., Gu, Z. and Yue, Z.(1993b)Fluorescent chromosome banding in six taxa of Eurasian Larix, Pinaceae. La Kromosomo II-69: 2342-2354.
Hizume, M., Yamasaki, Y., Kondo, K., Yang, Q., Hong, D. and Tanaka, R.(1994b)Fluorescent chromosome bandings in two Chinese varieties of Larix gmelinii, Pinaceae. La Kromosomo II-74: 2563-2570.
Hong, D. ed.(1989)Plant Chromosome Research 1987. Organizing committee of the Sino-Japanese Symposium on Plant Chromosomes. pp 437.
Mehra, P. N. and Khoshoo, T. N.(1956)Cytology of conifers. I, II. J. Genet. 54: 165-180, 181-185.
Sax, K. and Sax, H.J.(1933)Chromosome number and morphology in conifers. J. Arnold Arbor. 14: 356-375.
Murray, B. G.(1998)Nuclear DNA amount in gymnosperms. Ann. Bot. 82(Suppl. A): 3-15.
Ohri, D. and Khoshoo, T.N.(1986)Genome size in gymnosperms. Plant Syst. Evol. 153: 116-132.
Tanaka, R. ed.(1994). Plant Chromosome Research 1992. International Academic Publishers, Beijing, 482p.
Tanaka, R. and Hizume, M.(1980)C banding treatment for the chromosomes of some gymnosperms. Bot. Mag. Tokyo 93: 167-170.

Abstract

Summary: In gymnosperms chromosomes were analyzed by fluorescence banding using DAPI and chromomycin As and in situ hybridization with some probes in addition to conventional cytogenetic technique to be revealed as follows; Several valuable information on chromosomes in Chinese conifers such as comparative FISH analysis in Picea koreiensis and P. crassifolia. In Larix species examined were divided into six groups by fluorescence banding pattern and the DNA sequence localizing at the proximal DAPI-band was analyzed to be a tandem repeated AT-rich sequence and the sequence was presented in all Larix species examined. Sex chromosomes were discovered in Podocarpus macrophyllus and Cycas revoluta. In Pinus densiflora and P. thunbergii the DNA sequences localized at the fluorescent bands were revealed. The multi color FISH using four DNA probes allows to be identified each chromosome in each species and homoeologous chromosomes between these species.

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