Somaclonal variation and the ploidy level in Phalaenopsis alliance

Tian-Su Zhou; Shuichi Sakaguchi

doi:10.5685/plmorphol.15.2

Tian-Su Zhou, Shuichi Sakaguchi

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Keywords: DNA content, flow cytometry mass propagation, orchid, protocorm-like-body

JOURNAL FREE ACCESS

2003 Volume 15 Issue 1 Pages 2-7

DOI https://doi.org/10.5685/plmorphol.15.2

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Published: 2003 Received: December 18, 2003 Available on J-STAGE: June 28, 2010 Accepted: - Advance online publication: - Revised: -
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Date of correction: June 28, 2010 Reason for correction: - Correction: PDF FILE Details: -

Date of correction: June 28, 2010 Reason for correction: - Correction: KEYWORD Details: Wrong : Key words: DNA content, flow cytometry mass propagation, orchid, protocorm-like-body
Right : DNA content, flow cytometry mass propagation, orchid, protocorm-like-body

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Abstract

Summary: We tested the fbrmation of malfbrmed plantlets that are usually fbund during mass propagation in Phalaenopsis alliance(including Doritaenopsis). When 75 normal and malfbrmed sections of protocorm-like-bodies(PLB)of Doritaenopsis City Girl were cultured in vitro for mass propagation, 1.7%and100% of malfbrmed plantlets were formed respectively, Cytometric analysis revealed that the malfbrmed plantlets had double the nuclear DNA content in comparison to normal ones. They were 8C and 4C amounts in comparison with the 2C internal standard of Phalaenopsis schilleriana. Malformed plantlets also showed larger nuclei than normal ones in apical meristems of root tips and in the epidermis of PLB and leaves in 4, 6-diamidino-2-phenylindole(DAPI)-stained sections. Analyses by light microscopy and confocal laser microscopy suggested that the formation of malfbrmed PLB was possibly due to changes of 4C cells to8C cells in the epidermis.

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