Abstract
Summary: To determine how, where and when nano-machines and/or nano-systems act within cells is necessary for understanding life phenomena. To analyze the cellular structure at nano level, electron microscope is necessary. However, until recently, the analysis of cellular structure by electron microscopy has been limited by both specimen preparation method and by image analysis problems. Both of these limitations can be overcome by combining cryofixation methods in conjunction with electron tomography. In this lecture, we will describe the history and the method of electron tomography.