PLANT MORPHOLOGY
Online ISSN : 1884-4154
Print ISSN : 0918-9726
ISSN-L : 0918-9726
THREE-DIMENSIONAL DISTRIBUTION OF LHCP II APOPROTEIN IMMUNOREACTION IN SYNCHRONIZED CELLS OF EUGLENA
Tetsuaki Osafune
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1991 Volume 3 Issue 1 Pages 49-55

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Abstract
Euglena gracilis Z cells were synchronized under photoautotrophic conditions using 14h light: 10h dark regimen. The cells sampled in the middle the growth phase of the cell cycle, after 10hr in the light, were serially sectioned for immunoelectron microscopy. Sections were labelled with antibody raised against Euglena light harvesting chlorophyll apoprotein (LHCP II)followed by protein A-gold. Computer aided reconstruction of three-dimensional models revealed spatial distribution of LHCP II in the early growth phase of the cell cycle, and confirmed that LHCP II was highly concentrated over the Golgi apparatus and chloroplast membranes in Euglena.
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© The Japanese Society of Plant Morphology
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