Analysis of microtubule distribution in root tip cells of Allium cepa L. using a confocal laser scanning microscope

Yoshinobu MINEYUKI

doi:10.5685/plmorphol.5.127

Yoshinobu MINEYUKI

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Keywords: Allium cepa, , , ,, confocal laser scanning microscope, microtubule, preprophase band, root meristems

JOURNAL FREE ACCESS

1993 Volume 5 Issue 2 Pages 127-133

DOI https://doi.org/10.5685/plmorphol.5.127

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Published: 1993 Received: November 05, 1993 Available on J-STAGE: June 28, 2010 Accepted: - Advance online publication: - Revised: -
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Date of correction: June 28, 2010 Reason for correction: - Correction: PDF FILE Details: -

Date of correction: June 28, 2010 Reason for correction: - Correction: DTRECEIVED Details: Right : 19931105

Date of correction: June 28, 2010 Reason for correction: - Correction: KEYWORD Details: Wrong : Key words: Allium cepa, confocal laser scanning microscope, microtubule, preprophase band, root meristems
Right : Allium cepa, , , ,, confocal laser scanning microscope, microtubule, preprophase band, root meristems

Date of correction: June 28, 2010 Reason for correction: - Correction: CITATION Details: Wrong : E
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Abstract

In order to see whether a confocallaser scanning microscope(CLSM)is a useful tool for studies on the microtubule(MT)organization in plant cells, the spatial distribution of MTs in root tip cells of Allium cepa L. was examined using a CLSM. As the resolution of the image is improved using a CLMS, we could clearly detect MTs connecting between the spindle and the preprophase band(PPB)at late prophase stage. Although it was not easy to analyze the developmental process of spindle MTs on the nuclear surface using a conventional fluorescent microscope because of the strong flourescent signal from the PPB, a stereo pair image reconstructed from a focal series of images enabled us to see the detailed process of MT organization on the nuclear surface. Based on these results, the utility and problems of CLSM are discussed.

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