Morphological changes in Golgi apparatus induced by brefeldin A treatment in tobacco protoplasts

Takako S. Kaneko; Rei Watanabe; Mamiko Sato; Masako Osumi; Akira Takatsuki

doi:10.5685/plmorphol.6.1

Takako S. Kaneko, Rei Watanabe, Mamiko Sato, Masako Osumi, Akira Takatsuki

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Keywords: Nicotiana tabacum L. var. Xanthi, tobacco cells, tobacco protoplasts, Golgi apparatus, brefeldin A

JOURNAL FREE ACCESS

1994 Volume 6 Issue 1 Pages 1-11

DOI https://doi.org/10.5685/plmorphol.6.1

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Published: 1994 Received: September 13, 1994 Available on J-STAGE: June 28, 2010 Accepted: - Advance online publication: - Revised: -
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Date of correction: June 28, 2010 Reason for correction: - Correction: PDF FILE Details: -

Date of correction: June 28, 2010 Reason for correction: - Correction: KEYWORD Details: Wrong : Key Words: Nicotiana tabacum L. var. Xanthi; tobacco cells; tobacco protoplasts; Golgi apparatus; brefeldin A.
Right : Nicotiana tabacum L. var. Xanthi, tobacco cells, tobacco protoplasts, Golgi apparatus, brefeldin A

Date of correction: June 28, 2010 Reason for correction: - Correction: CITATION Details: Wrong : E
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Abstract

We investigated the effect of brefeldin A(BFA)on the Golgi apparatus in cultured tobacco protoplasts. Within 3 hr of treatment with brefeldin A(10μg/ml), the diameter of the Golgi cisternae became about four times greater than the control. The expanded cisternae were loosely organized, their tips were sprinkled with ribosomes and the distance between the cisternae increased. No other significant morphological changes were detected, except the appearance of many vacuoles. From these observations it was concluded that BFA causes morphological changes in Golgi apparatus.

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