Abstract
Two convenient methods for observation of cells at the surface of plant organs, are introduced. The first method is negative staining of organ surface with water-insoluble dye, which is followed by observation with binocular dissecting microscope. In this method boundaries between the cells are visualized by the accumulation of pigment of waterproof felt-tip pens or orcein dissolved in propylene glycol α-monomethyl ether. The second method is the replica-SEM method, in which replicas of plant organs made of epoxy resin are observed by a scanning electron microscope: the molds for the replicas are prepared using dental impression polymer. These methods have been originally developed in USA and Australia and some adaptations for Japanese users are described.
