Plant Production Science
Online ISSN : 1349-1008
Print ISSN : 1343-943X
Crop Morphology
Structural and Functional Differentiation of Bundle Sheath and Mesophyll Cells in the Lamina Joint of Rice Compared with that in the Corresponding Region of the Liguleless Genotype
Koichi TsutsumiMichio KawasakiMitsutaka TaniguchiTomio ItaniMasahiko MaekawaHiroshi Miyake
Author information

2007 Volume 10 Issue 3 Pages 346-356


The structural and functional characterization of the blade-sheath boundary region of a rice cultivar T65 and its near-isogenic line T65lg were examined by light and electron microscopy and in situ hybridization. Starch accumulation in bundle sheath cells was compared between the lamina joint of T65 and the corresponding region of T65lg and also between the lamina joint and the leaf blade. In the lamina joint of T65, starch grains were predominantly accumulated in bundle sheath cells, and the starch-containing chloroplasts within these cells were spherical in shape. On the other hand, in the blade-sheath transition region of T65lg, little starch accumulation was observed and the chloroplasts were oval in both mesophyll and bundle sheath cells. Furthermore, photosynthesis-related genes, rbcS and cab, were expressed in mesophyll cells within the blade-sheath transition region of T65lg as in the leaf blade and sheath, while no expression of these genes was found within the lamina joint of T65. These facts indicate that T65lg can not develop the lamina joint from either structural or functional aspect. The present results suggest that the control mechanism of starch accumulation in bundle sheath chloroplasts in the lamina joint differs from that in leaf blade in rice.

Information related to the author

This article cannot obtain the latest cited-by information.

© 2007 by The Crop Science Society of Japan
Previous article Next article