Abstract
Polyamines acid extracted from roots, stems, leaves, flower buds, flowers and seeds of eight Brassica crop plants (cabbage, broccoli, cauliflower, Komatsuna, Chingensai, turnip, Aburana and Seiyou-aburana) with or without clubroot disease were analyzed by high-performance liquid chromatography and gas chromatography. Endogenous concentrations of polyamines per wet weight of the organ were calculated. In cabbage, broccoli and Komatsuna clubroot galls, the levels of putrescine, spermidine and spermine were increased whereas the levels of agmatine levels were decreased after the infection with a protist, Plasmodiophora brassicae. The levels of 2-phenylethylamine and homospermidine found in the normal healthy roots were decreased in the clubroots. The thermospermine level in broccoli was higher in the sprout stem than in the sprout root or other organs. A high agmatine level was found in the healthy sprouts and flower buds of broccoli and cauliflower flower buds. Diaminopropane, cadaverine and norspermine were detected in some Brassica organs as a minor polyamine. The seeds of the eight Brassica plants were rich in spermine and spermidine.
