FORMATION OF POLY-L-ARGININE - HYDROXYAPATITE NANOPARTICLE COMPLEXES AND INTERACTIONS BETWEEN COMPLEXES AND LIPIDBILAYER MEMBRANES.

Abstract

A certain molecule permeable to a phospholipid bilayer membranes (cell or endosormal membrane ) is a useful carrier (i.e. vector) for drugs (especially polymeric drugs). We prepared two types of the hydroxyapatite nanoparticle - poly-L-arginine complexes in a different solvent, which were used as the probes for the study on the translocationability through the negatively charged phospholipid bilayer membranes by means of a confocal laser scanning microscope (CLSM). CLSM observation and secondary structure of poly-L-arginine moieties which estimated by FT-IR measurement showed the transformation of the polypeptide secondary structure on the surface of liposome is on important key step of membrane transfer of the these complexes.