PHOSPHORYLATION OF CITRULLINE WITH CYCLO-TRIPHOSPHATE IN AQUEOUS SOLUTION

Hideko Maeda; Tomoaki Kita; Takayuki Iiduka; Mitsutomo Tsuhako; Hiroyuki Nariai; Hirokazu Nakayama

doi:10.3363/prb.28.6

Hideko Maeda, Tomoaki Kita, Takayuki Iiduka, Mitsutomo Tsuhako, Hiroyuki Nariai, Hirokazu Nakayama

Author information

Keywords: Phosphorylation, Citrulline, cyclo-Triphosphate, HPLC, ³¹P NMR

JOURNAL FREE ACCESS

2013 Volume 28 Pages 6-9

DOI https://doi.org/10.3363/prb.28.6

Details

Abstract

Phosphorylation of citrulline (Cit) has been achieved using inorganic cyclo-triphosphate (P_3m) in aqueous solution. The optimum condition for the phosphorylation of Cit with P_3m is Cit : P_3m = 1.75 M : 0.5 M, pH 12 and 10°C. N²-(Phosphono)citrulline was synthesized with the yield of more than 85 %. The reaction mechanism of Cit with P_3m was discussed.

Corresponding author

Register with J-STAGE for free!