Abstract
Phosphorylation of theanine (Thea) has been achieved using inorganic cyclo-triphosphate (P3m) in aqueous solution. The optimum condition for the phosphorylation of Thea with P3m is Thea : P3m = 2.0 M : 0.5 M, pH 11 and 10 °C. N2-(Phosphono)theanine was synthesized with the yield of more than 90 % and then remained constant after 40 d without hydrolysis of the monophosphate derivative. The phosphorylated product of Thea has a N-P bond in the molecule. The reaction mechanism of Thea with P3m was discussed.
