Phosphorus Research Bulletin
Online ISSN : 1882-2363
Print ISSN : 0918-4783
ISSN-L : 0918-4783
DEMINERALIZED DENTIN MATRIX WITH ARTIFICIAL PERFORATIONS FOR BONE REGNERATION IN CRITICAL DEFECTS OF ADULT SHEEP
ARAFAT KABIRMASARU MURATATOSHIYUKI AKAZAWAKATSUHUSA YAMADAMANABU ITOTAKANORI SHIBATA
Author information
JOURNAL FREE ACCESS

2016 Volume 31 Pages 1-3

Details
Abstract
A vital tooth-derived demineralized dentin matrix (DDM) is defined as acid-insoluble, cross-linked collagen with growth factors. The objective of this study is to evaluate the feasibility of the perforated-DDM for bone regeneration. Thirty perforations were added to the human vital wisdom tooth after the removal of enamel portion. The modified tooth was partially demineralized in 0.34 N HNO3 for 30 min and grafted into the critical defect of sheep iliac to evaluate bone regeneration. Sheep were sacrificed for μ-CT and histological evaluation at 2 and 4 months after the graft. New bone was connected directly with the grafted DDM at 2 months. The volume of new bone was comparable with the μ-CT, 3D, and the histological findings at 2 and 4 months. The amount of new bone tissue in the critical defects at 4 months was higher than that at 2 months. The results revealed that the human tooth-derived block material with artificial porous structure, the perforated-DDM, successfully contributed to bone ingrowth as the unique scaffold. This novel human tooth-derived scaffold may be a promising biomaterial to induce bone regeneration in the tri-cortical critical-sized bone defects.
Content from these authors
© 2016 Japanese Association of Inorganic Phosphorus Chemistry
Previous article Next article
feedback
Top