Abstract
On-line analysis of organic phosphorus compounds with P-O-C and P-C bonds have been developed based on HPLC and two-step postcolumn reactions. The calibration curve for the organic phosphorus compounds showed good linearity (correlation coefficient>0.9918). The standard deviations (n=9) of the method were smaller than 0.91%. The detection limits (S/N=2) of the organic phosphorus compounds were ca. 0.5 nmol as the injected amount. This method could be applied to the determination of the organic phosphorus compounds in the 5-100 μM range (injection volume, 100 μl).
