2003 Volume 16 Pages 11-18
The phosphorylation of maltotriose by cyclo-triphosphate (P3m) proceeded on not only 1-OH group of a reducing unit, but also 2′-OH and 2″-OH of nonreducing units in aqueous solution. The optimum condition for the phosphorylation of maltotriose with P3m was a molar ratio of P3m: maltotriose =1: 4, pH 12, and 25°C.