Abstract
A number of researchers have used a simulated body fluid (SBF) to get an in vitro preliminary assessment of bone-bonding ability of an artificial material by examining apatite-forming ability in the SBF, before animal experiments. However, the common method for preparing the SBF and for detecting an apatite formed on a surface of a material in the SBF has not been established yet. The ion concentrations of the conventional SBF are not exactly equal to those of human blood plasma. In the present study, the detailed preparing method, and a newly improved SBF were proposed. In vitro assessments of apatite-forming ability in the conventional and proposed SBFs were performed.
