2005 Volume 18 Pages 55-64
Hydroxyapatite was formed by the extremely slow addition of diammonium hydrogenphosphate to a solution of calcium nitrate at pH9 buffered with ethylenediamine at 36.5°C. The Ca/P molar ratio in the precipitate yielded was measured by inductively coupled plasma atomic emission spectrometry and the condition of the crystals was examined by X-ray diffraction. This synthetic apatite was compared with human tooth and bones from cattle and pigs. The crystallization condition and Ca/P molar ratio were extremely similar to those of human tooth.