ADSORPTION OF BOVINE SERUM ALBUMIN ON AMORPHOUS CALCIUM PHOSPHATE IN RINGER SOLUTION

Abstract

The present study was made to investigate adsorption of bovine serum albumin (BSA) on hydroxyapatite (HAp) which was crystallized by dipping amorphous calcium phosphate (ACP) in Ringer solution having a composition similar to body fluid in vivo. Characterization of products was determined by means of X-ray diffraction, ion chromatography and ultraviolet spectrophotometry. When ACP was dipped for 48 hours in Ringer solution, maximum amount of BSA adsorbed on HAp prepared by crystallizing ACP was 190mg/g. Adsorption velocity till reaching to maximum amount of BSA adsorbed on HAp was speeded up by increasing concentration of BSA. The equilibrium amount of BSA adsorbed on HAp was 1.2mg/m². When ACP was dipped in Ringer solution, the largest quantity of BSA could be adsorbed to HAp crystallized in a short time.