Abstract
It was investigated to treat the wastewater containing phenol and salicylate using both phenol degrading bacteria and recombinant degrading phenol and salicylate immobilized in polyvinyl alcohol (PVA). Immobilization of living cells was suggested to mitigate the inhibiting action of phenol. Using immobilized phenol degrading bacterial cells, high phenol loading operation could be achieved in comparison with suspended cells or activated sludge such as 3.0g/l·day of volumetric loading. Salicylate degrading gene from NAH plasmid was transformed into phenol degrading bacteria and recombinant degrading both phenol and salicylate was breeded. Using activated sludge with recombinant cells immobilized in PVA, wastewater containing both phenol and salicylate could be treated effectively. Stability of plasmid in the recombinat was also investigated under conditions with or without selection pressures. It was concluded that immobilization of special kinds of living cells such as phenol degrading bacteria and/or recombinants containing aromatic compounds degradation-genes has many advantages for wastewater treatment.
