Abstract
Batch-cultivation experiments were carried out in order to investigate the utilization of various fatty acids and hydrocarbons for Nocardia amarae from foaming activated sludge. The optimum ultrasonic wave time was determined to be 2 minutes for the measurement of vial cell count and OD600. It was shown that both low-class fatty acids (C2-C5) and pharaffinic hydrocarbons (C12-C20) were utilized by N. amarae, and that the highest specific growth rate was obtained in case of octadecane (C18), which was 5.20 (1/day). It was observed that the different flocs of N. amarae were formated in accordance with the medium compositions, in particular that the floc in case of hexadecane was the disk type and about 5mm in diameter, which was formated at the hexadecane-solution interface. As the above-mentioned results, the capability of applying the octadecane and/or hexadecane medium to the colony counting method was suggested.
